abstract：:In the present study, we examined the effect of the histone deacetylase (HDAC) inhibitors trichostatin A (TSA), valproic acid (VA) and sodium-butyrate on the metamorphosis of larvae of the human blood-fluke Schistosoma mansoni from the free-swimming miracidia into the intramolluskal sporocyst. We show that HDAC inhibi...

journal_title：Experimental parasitology

authors： Azzi A,Cosseau C,Grunau C

更新日期：2009-03-01 00:00:00

abstract：:Interleukin-13 (IL-13) is a powerful anti-inflammatory cytokine that was previously shown to be a susceptibility factor for Leishmania major (L. major) infection. In this study we report a different role for IL-13 in rats infected with L. major; rIL-13 stimulates expression of pro-inflammatory cytokines and IL-12 whic...

journal_title：Experimental parasitology

authors： Haber ME,Daher CF,Karam MC,Baroody GM

更新日期：2009-03-01 00:00:00

abstract：:Toxoplasma gondii is the etiologic agent of toxoplasmosis. Although the combination of sulfadiazine and pyrimethamine is used as therapy for this disease, these drugs can have serious side effects and its use is limited in pregnancy. Therefore there is a need for new anti-T. gondii drugs in the clinic. Some systems fo...

journal_title：Experimental parasitology

authors： Jin C,Kaewintajuk K,Jiang J,Jeong W,Kamata M,Kim HS,Wataya Y,Park H

更新日期：2009-02-01 00:00:00

abstract：:In the present study, samples representing Orientobilharzia turkestanicum from cattle, sheep, cashmere goat and goat in Heilongjiang Province, China, were characterized and grouped genetically by sequences of internal transcribed spacer (ITS, including ITS-1 and ITS2) and 28S ribosomal DNA (28S rDNA). The ITS and 28S ...

journal_title：Experimental parasitology

authors： Wang CR,Li L,Ni HB,Zhai YQ,Chen AH,Chen J,Zhu XQ

更新日期：2009-02-01 00:00:00

abstract：:Reliable analytical techniques to test growth-promoting and antimalarial efficacy on plasmodia are very important. Flow cytometry (FCM) offers the possibility to study developmental stages of intraerythrocytic growth of malaria parasites using nucleic acid staining. To analyze the growth of Plasmodium falciparum SYBR ...

journal_title：Experimental parasitology

authors： Izumiyama S,Omura M,Takasaki T,Ohmae H,Asahi H

更新日期：2009-02-01 00:00:00

abstract：:Opisthorchis viverrini is the only liver fluke that has been proved to be associated with cholangiocarcinoma (CCA). However, the mechanisms by which O. viverrini participates in the carcinogenesis of CCA are still unclear. To understand the biology and host-parasite interaction related to O. viverrini infection, gene ...

journal_title：Experimental parasitology

authors： Chutiwitoonchai N,Shen Y,Zheng H,Xiong H,Zhao G,Imtawil K,Intapan PM,Wongkham S,Wongkham C

更新日期：2008-12-01 00:00:00

abstract：:Through a continuous in vivo drug pressure protocol, using mice as experimental model, we induced benznidazole resistance in Trypanosoma cruzi stocks. Full resistance was obtained for four out of five T. cruzi stocks analyzed. However, the number of benznidazole doses (40-180), as well as the time (4-18 months) necess...

journal_title：Experimental parasitology

authors： Dos Santos FM,Caldas S,de Assis Cáu SB,Crepalde GP,de Lana M,Machado-Coelho GL,Veloso VM,Bahia MT

更新日期：2008-12-01 00:00:00

abstract：:Live adult and juvenile entomopathogenic Steinernema carpocapsae DD136 (P. Nematoda) were not subjected to adhesion by haemocytes of lepidopteran insect larvae of Galleria mellonella or Malacosoma disstriain vitro or in vivo. In vitro freeze-killed nematodes exhibited haemocyte attachment, the intensity increasing wit...

journal_title：Experimental parasitology

authors： Walter TN,Dunphy GB,Mandato CA

更新日期：2008-10-01 00:00:00

abstract：:Nitric oxide (NO) has diverse biological functions. Numerous studies have documented NO's biosynthetic pathway in a wide variety of organisms. Little is known, however, about NO production in intraerythrocytic Plasmodium falciparum. Using diaminorhodamine-4-methyl acetoxymethylester (DAR-4M AM), a fluorescent indicato...

journal_title：Experimental parasitology

authors： Ostera G,Tokumasu F,Oliveira F,Sa J,Furuya T,Teixeira C,Dvorak J

更新日期：2008-09-01 00:00:00

abstract：:Basic aspects of the generation, structure and function of Echinococcus granulosus protoscoleces are unknown. We review the work done on the structure and ultrastructure of the E. granulosus protoscolex and provide new data together with a comprehensive view of this form of the parasite. The surface, as observed by sc...

journal_title：Experimental parasitology

authors： Galindo M,Schadebrodt G,Galanti N

更新日期：2008-08-01 00:00:00

abstract：:In this work CD4-knockout mice were used as a model to analyse the role of CD4+ T cells in the antibody response against Echinococcus granulosus immunization or experimental infection. Results obtained with mice immunized with protoscolex antigens indicated that these contain T-independent antigens. After infection, C...

journal_title：Experimental parasitology

authors： Baz A,Carol H,Fernández V,Mourglia-Ettlin G,Nieto A,Orn A,Dematteis S

更新日期：2008-08-01 00:00:00

abstract：:The presence of the cytoskeleton of Acanthamoeba castellanii was observed by means of cryo-electronmicroscopy and immunofluorescence techniques. This structure is formed largely by fibers and networks of actin located mainly in cytoplasmic locomotion structures as lamellipodia and as well as in various endocytic struc...

journal_title：Experimental parasitology

authors： González-Robles A,Castañón G,Hernández-Ramírez VI,Salazar-Villatoro L,González-Lázaro M,Omaña-Molina M,Talamás-Rohana P,Martínez-Palomo A

更新日期：2008-07-01 00:00:00

abstract：:The schistosomicidal activity of praziquantel (PZQ) is accompanied by a large influx of calcium into the worms, suggesting that this phenomenon could be the source of the observed muscular contraction, surface disruption and eventual death of the parasite. We have incubated live adult schistosomes in a medium containi...

journal_title：Experimental parasitology

authors： Pica-Mattoccia L,Orsini T,Basso A,Festucci A,Liberti P,Guidi A,Marcatto-Maggi AL,Nobre-Santana S,Troiani AR,Cioli D,Valle C

更新日期：2008-07-01 00:00:00

abstract：:Toxocara canis is a dog helminth which causes visceral larva migrans (VLM) when infecting humans as a larva. The infection is demonstrated by detecting IgG antibodies against excretory-secretory larval antigens (ESLA) in serum by ELISA. The production of ESLA involves the collection of adult worms from dog puppy stool...

journal_title：Experimental parasitology

authors： Alcântara-Neves NM,dos Santos AB,Mendonça LR,Figueiredo CA,Pontes-de-Carvalho L

更新日期：2008-07-01 00:00:00

abstract：:Aedes albopictus mosquito is an opportunistic blood feeder and has a broad host range. The feeding behavior and habits of this mosquito are liable to increase the transmission potential of arboviruses. The survival and fecundity in A. albopictus fed on different hosts and post-blood meal provision of sugar were invest...

journal_title：Experimental parasitology

authors： Xue RD,Ali A,Barnard DR

更新日期：2008-06-01 00:00:00

abstract：:The cDNA of a Schistosoma japonicum myophilin-like protein was cloned, sequenced, and expressed in Escherichia coli as a recombined protein (rSj myophilin-like protein), and the protein was purified by affinity chromatography. The deduced amino acid sequences of the Sj myophilin-like protein showed significant homolog...

journal_title：Experimental parasitology

authors： Peng H,Song K,Huang C,Ye S,Song H,Hu W,Han Z,McManus DP,Zhao G,Zhang Q

更新日期：2008-05-01 00:00:00

abstract：:In this work we demonstrated that promastigotes of Leishmania amazonensis exhibit an Mg-dependent ecto-ATPase activity, which is stimulated by heat shock. The Mg-dependent ATPase activity of cells grown at 22 and 28 degrees C was 41.0+/-5.2 nmol Pi/h x 10(7)cells and 184.2+/-21.0 nmol Pi/h x 10(7)cells, respectively. ...

journal_title：Experimental parasitology

authors： Peres-Sampaio CE,de Almeida-Amaral EE,Giarola NL,Meyer-Fernandes JR

更新日期：2008-05-01 00:00:00

abstract：:The tegumental alterations in adult Fasciola hepatica induced by the experimental fasciolide OZ78 were investigated utilizing scanning electron microscopy (SEM). Twelve weeks post-infection with F. hepatica, rats were treated with a single 100mg/kg oral dose of OZ78 and flukes were recovered from the bile ducts after ...

journal_title：Experimental parasitology

authors： Keiser J,Morson G

更新日期：2008-05-01 00:00:00

abstract：:Entomopathogenic nematodes complete their life cycles inside dead insects. The emergence of new infective juveniles from the cadaver has been attributed (but never demonstrated) to food depletion or to the accumulation of metabolites from the breakdown of the host's tissues. Here we give evidence that emergence is tri...

journal_title：Experimental parasitology

authors： San-Blas E,Gowen SR,Pembroke B

更新日期：2008-05-01 00:00:00

abstract：:Given that signal sequences between secreted proteins of different species can be interchanged, it is reasonable to expect that both mammalian and yeast signal sequence trapping (SST) systems would secrete Haemonchus contortus proteins with similar efficiency and quality. To determine if H. contortus cDNAs that contai...

journal_title：Experimental parasitology

authors： Donald D,Chen Y,Hartman D,Zawadzki JL

更新日期：2008-05-01 00:00:00

abstract：:Chagas disease is characterized by cardiac lesions and a high level of PGE2. Our objective was to investigate the role of PGE2 in cardiac lesions. BALB/c mice were infected with Trypanosoma cruzi (1x10(3) trypomastigote forms) and were treated daily with PBS, meloxicam (0.5 mg/kg) or etoricoxib (0.6 mg/kg). The animal...

journal_title：Experimental parasitology

authors： Abdalla GK,Faria GE,Silva KT,Castro EC,Reis MA,Michelin MA

更新日期：2008-04-01 00:00:00

abstract：:Only a few drugs are available for chemotherapy of African trypanosomiasis and there is an urgent need for the development of new anti-trypanosomal agents. In this study, the anti-helminthic drug niclosamide was tested for its trypanocidal activity in vitro using culture-adapted bloodstream forms of Trypanosoma brucei...

journal_title：Experimental parasitology

authors： Merschjohann K,Steverding D

更新日期：2008-04-01 00:00:00

abstract：:Insects manifest effective immune responses that include both cellular and humoral components. Morphological and quantitative aspects of cellular and humoral cooperation during nodule formation in Chrysomya megacephala hemolymph against Saccharomyces cerevisae yeast cells were demonstrated for the first time. The anal...

journal_title：Experimental parasitology

authors： Faraldo AC,Gregório EA,Lello E

更新日期：2008-03-01 00:00:00

abstract：:The protozoan Trypanosoma evansi is described as presenting high morphological and genetic similarities among the isolates despite its biological heterogeneity and wide geographical distribution. PCR amplification of the internal transcribed spacers of the ribosomal gene in combination with the coding region of the 5....

journal_title：Experimental parasitology

authors： de Oliveira Lima AN,da Silva Santos S,Herrera HM,Gama C,Cupolillo E,Jansen AM,Fernandes O

更新日期：2008-03-01 00:00:00

abstract：:Though parasite cyclophilins are promising new drug targets, Eimeria tenella cyclophilins have not been characterized yet. Here, we describe an 89kDa cyclophilin, designated EtCYP89. It is expressed throughout the developmental cycle of E. tenella, both in the intracellular stages in chicken and in extracellular sporu...

journal_title：Experimental parasitology

authors： Hosse RJ,Krücken J,Bierbaum S,Greif G,Wunderlich F

更新日期：2008-02-01 00:00:00

abstract：:Nitric oxide (NO), derived from catalysis of inducible NO synthase (iNOS), limits malaria parasite growth in mammals. Transforming growth factor (TGF)-beta1 suppresses iNOS in cells in vitro as well as in vivo in mice, but paradoxically severe malaria in humans is associated with low levels of TGF-beta1. We hypothesiz...

journal_title：Experimental parasitology

authors： Luckhart S,Lieber MJ,Singh N,Zamora R,Vodovotz Y

更新日期：2008-02-01 00:00:00

abstract：:Toxoplasma gondii is responsible for fetopathy in farm animals and humans and severe disease in immunocompromised individuals (i.e. AIDS patients). Effective vaccines, inducing protective and long-lasting immunity to this global parasite, are still desired. In the work, we evaluated the immunogenic and immunoprotectiv...

journal_title：Experimental parasitology

authors： Gatkowska J,Gasior A,Kur J,Dlugonska H

更新日期：2008-02-01 00:00:00

abstract：:BmSI-7 and BmSI-6, two Boophilus microplus subtilisin inhibitors (BmSI) were purified and characterized from eggs. The inhibitors isolated by classical purification methods presented molecular masses of 7408 and 7271Da, respectively, by MALDI-TOF-MS. Both BmSI-7 and BmSI-6 inhibited neutrophil elastase (K(i) 0.4 and 0...

journal_title：Experimental parasitology

authors： Sasaki SD,de Lima CA,Lovato DV,Juliano MA,Torquato RJ,Tanaka AS

更新日期：2008-02-01 00:00:00

abstract：:In this report, an immunodominant antigen called BgIRA from Babesia gibsoni is identified and described. A highly repetitive antigen was screened from a cDNA library. The genomic BgIRA gene exists as single cope gene and contains 10 introns. BgIRA plays a dominant role in the immune response in dogs infected with B. g...

journal_title：Experimental parasitology

authors： Jia H,Terkawi MA,Aboge GO,Goo YK,Zhou J,Lee EG,Nishikawa Y,Igarashi I,Fujisaki K,Xuan X

更新日期：2008-01-01 00:00:00

abstract：:An increased level of plasma corticosterone is one manifestation of severe environmental or physiologic stress. The stress response mediated by the hypothalamic-pituitary-adrenal axis is already known to suppress immunoglobulin production and to impair immune function, but there are few studies relating stress and pla...

journal_title：Experimental parasitology

authors： Santos CD,Prado JC Jr,Toldo MP,Levy AM,Franci CR,Caldeira JC

更新日期：2007-12-01 00:00:00

abstract：:One difficulty facing post-genomic analyses of schistosomes is the limited data on sites of expression of many gene products expressed by the parasites in their hosts. The potential for use of laser microdissection microscopy as a preparative technique for transcriptional and proteomic profiling is reviewed. This tech...

journal_title：Experimental parasitology

authors： Jones MK,Higgins T,Stenzel DJ,Gobert GN

更新日期：2007-11-01 00:00:00

abstract：:In this study, a survey was conducted in order to determine the presence and pathogenic potential of free-living amoebae of Acanthamoeba genus in a population of Barbary Ground squirrels in Fuerteventura Island (Canary Islands, Spain) and Morocco. Identification of Acanthamoeba was based on the morphology of cyst and ...

journal_title：Experimental parasitology

authors： Lorenzo-Morales J,López-Darias M,Martínez-Carretero E,Valladares B

更新日期：2007-09-01 00:00:00

abstract：:This study presents the first genetic characterization of five Trypanosoma rangeli isolates from Minas Gerais, in the southeast of Brazil and their comparison with Colombian populations by minicircle classification, RAPD-PCR and LSSP-PCR analyses. Our results demonstrated a homogenous T. rangeli population circulating...

journal_title：Experimental parasitology

authors： Marquez DS,Ramírez LE,Moreno J,Pedrosa AL,Lages-Silva E

更新日期：2007-09-01 00:00:00

abstract：:It is well known that Anopheles dirus is naturally refractory to rodent malaria parasite, Plasmodium yoelii, but the mechanism is still largely unknown. Here, we found that some P. yoelii taken into An. dirus could develop into oocysts, but oocysts were partially melanized at 7 days and completely melanized at 15 days...

journal_title：Experimental parasitology

authors： Wen-Yue X,Jian Z,Tao-Li Z,Fu-Sheng H,Jian-Hua D,Ying W,Zhong-Wen Q,Li-Sha X

更新日期：2007-08-01 00:00:00

abstract：:The kinetics of specific immunoglobulin M, A and IgG subclasses against Echinostoma caproni (Trematoda: Echinostomatidae) were analyzed in serum and intestinal fluid of two host species (Wistar rats and ICR mice) in which the course of the infection markedly differs. In rats, the worms were rapidly expelled, whereas E...

journal_title：Experimental parasitology

authors： Sotillo J,Muñoz-Antoli C,Marcilla A,Fried B,Guillermo Esteban J,Toledo R

更新日期：2007-08-01 00:00:00

abstract：:In vitro anthelmintic activity of crude extracts of the ripe fruits of Hedera helix was investigated on eggs and adult nematode parasites Haemonchus contortus. Aqueous extract of H. helix was also evaluated for in vivo anthelmintic activity at dose of 1.13 and 2.25 g/kg in sheep artificially infected with H. contortus...

journal_title：Experimental parasitology

authors： Eguale T,Tilahun G,Debella A,Feleke A,Makonnen E

更新日期：2007-08-01 00:00:00

abstract：:During its life cycle the malarial parasite Plasmodium forms three invasive stages which have to invade different and specific cells for replication to ensue. Invasion is vital to parasite survival and consequently proteins responsible for invasion are considered to be candidate vaccine/drug targets. Plasmodium perfor...

journal_title：Experimental parasitology

authors： Ecker A,Pinto SB,Baker KW,Kafatos FC,Sinden RE

更新日期：2007-08-01 00:00:00

abstract：:Certain model inhibitors exerted selective action against the catalytic activity of nuclear DNA topoisomerase II (TOPII) of Leishmania panamensis promastigotes. The second-generation fluoroquinolones enoxacin and ciprofloxacin exhibited extraordinarily high anti-parasite selectivity displaying 582- and 40-fold greater...

journal_title：Experimental parasitology

authors： Cortázar TM,Coombs GH,Walker J

更新日期：2007-08-01 00:00:00

abstract：:Crohn's disease (CD) is considered to be caused by a disorder of the immune system and helminth infections may interact with development of the disease. We induced colitis in mice by trinitrobenzenesulfonic acid (TNBS) and observed the effects of intraperitoneally injected eggs of Schistosoma japonicum on the course o...

journal_title：Experimental parasitology

authors： Mo HM,Liu WQ,Lei JH,Cheng YL,Wang CZ,Li YL

更新日期：2007-08-01 00:00:00

abstract：:Trypanosomatid protozoa lack consensus promoters for RNA polymerase (RNAP) II. However, the artificial insertion of the T7 promoter (P(T7)) and the tetracycline repressor into Trypanosoma brucei cell lines expressing T7RNAP allows P(T7)-driven gene expression to be tetracycline-inducible. These cell lines provide a mo...

journal_title：Experimental parasitology

authors： Yao C,Luo J,Hsiao CH,Donelson JE,Wilson ME

更新日期：2007-07-01 00:00:00